Research Paper Volume 12, Issue 12 pp 11500—11516

Long noncoding RNA LINC00963 induces NOP2 expression by sponging tumor suppressor miR-542-3p to promote metastasis in prostate cancer

Figure 6. Long noncoding RNA LINC00963 functions as a competing endogenous RNA (ceRNA) and sponges tumor suppressor miR-542-3p to upregulate NOP2. (A) Relative protein levels of NOP2 in DU 145 cells transfected with empty vector lentivirus or OE-miR-542-3p lentivirus. (B) Relative protein levels of NOP2 in DU 145 cells transfected with empty vector lentivirus or SH-LINC00963 Lentivirus. (C, D) NOP2 mRNA (C) in 293T cells and protein level in DU 145 cells and PC-3 cells (D) following knockdown of LINC00963 and/or inhibition of miR-542-3p. Mean ± SEM, ****P < 0.001.