Research Paper Volume 12, Issue 12 pp 11500—11516

Long noncoding RNA LINC00963 induces NOP2 expression by sponging tumor suppressor miR-542-3p to promote metastasis in prostate cancer

Figure 7. NOP2 promotes prostate cancer (PCa) cell progression through epithelial-mesenchymal transition (EMT) pathway. (A) Heat map of increased genes in DU 145 cells transfected with OE-NOP2 Lentivirus. (B) Gene enrichment analysis showed the signaling pathways activated or inhibited by NOP2 overexpression. (C) EMT related proteins (E-cadherin, N-cadherin, and vimentin) was examined by western blot in vector or OE-NOP2 Lentivirus transfected cells and the data were quantitated. (D) NOP2 expression in SH-NOP2 Lentivirus- and pcDNA-NOP2 Lentivirus–transfected DU145 cells was examined by RT-qPCR. (E) Transwell invasion assays were performed to determine the invasion of SH-NOP2 Lentivirus–or pcDNA-NOP2 Lentivirus–transfected DU 145 cells. (F) Survival time of PCa mice transfected with SH-NOP2 Lentivirus or pcDNA-NOP2 Lentivirus. (G) Transcript levels of NOP2 in normal prostate tissues and PCa tissues and differential Gleason scores. (H) Receiver operation characteristic (ROC) curve analysis of the association of NOP2 with the diagnosis of PCa (AUC = 0.701). Mean ± SEM, ***P < 0.005, ****P < 0.001.