Figure 4. Ubiquitin-conjugating enzyme E2T (UBE2T) enhances glioblastoma (GBM) invasion and migration via GRP78. (A) Transfection efficacy of GRP78 siRNA in LN229 and U251 cell lines was analysed via immunoblotting. (B, C) The effects of GRP78 siRNA on the invasion and migration of LN229 (B) and U251 (C) cells. (D) UBE2T was depleted in GRP78–over-expressing LN229 and U251 cells. Cell lysates were analysed via immunoblotting using the indicated antibodies. (E, F) The effects of UBE2T depletion on the invasion and migration of GRP78–over-expressing LN229 (E) and U251 (F) cells. (Error bars indicate the SEM of three independent experiments. Two-tailed Student’s t-test. *, P < 0.05; **, P < 0.01; ***, P < 0.001).