Figure 6. The function of Ubiquitin-conjugating enzyme E2T (UBE2T) was uncovered in vivo. (A, B) The effects of transfection with short hairpin RNA (shRNA) targeting UBE2T-1, treatment with temozolomide (TMZ, 200 μM) or both on the invasion and migration of LN229 (A) and U251 (B) cells. (C, D) LN229 cells transfected with ShUBE2T-1 or the control vector (Sh Vector) were subcutaneously injected (5 × 106 cells per mouse) into 6-week-old nude mice (n = 9). Representative images (left) (C) and quantitative analysis (right) (D) of subcutaneous tumour volumes. (E) Haematoxylin and eosin (HE) staining was performed to illustrate the tumour cytostructure. (F) Immunohistochemical staining of UBE2T, GRP78 and Ki-67 in ShUBE2T-1 and ShVector subcutaneous tumour samples (×100). Scale bar, 100 μm. (Error bars indicate the SEM of three independent experiments. Two-tailed Student’s t-test. *, P < 0.05; **, P < 0.01; ***, P < 0.001).