Figure 6. MDM2-mediated p53 degradation induces osteoblast differentiation in vitro. (A, B) p53 levels in non-OP patients and OP patients were measured by qRT-PCR and western blot; n=10 per group. (C) MDM2 expression in hMSCs was assessed by qRT-PCR analysis after different treatments. (D–E) p53 levels were measured by qRT-PCR and western blot in the three groups. (F) Osteogenic gene levels were measured by qRT-PCR. (G–H) Alizarin red-mediated calcium staining in hMSCs 21 days after transfection with different constructs. Scale bar = 10mm. Data are means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.