Research Paper Volume 12, Issue 11 pp 10381—10397

High circ-SEC31A expression predicts unfavorable prognoses in non-small cell lung cancer by regulating the miR-520a-5p/GOT-2 axis

Figure 1. The expression of circ-SEC31A predicted an unfavorable prognosis in non-small cell lung cancer (NSCLC) patients. (A) Heat map of all differentially-expressed circRNAs between normal and tumor tissues. (B) RT-qPCR showing the relative expression of the circ-SEC31A from 20 NSCLC tumor tissues and adjacent non-tumor tissues. Data are presented as mean ± SD; **p<0.01. (C) RT-qPCR detection showing the relative expression of circ-SEC31A in A549, PC9, H1650, and normal lung epithelial cells (BEAS-2B). Data are presented as mean ± SD; ***P<0.001 vs. the normal group. (D) The expression of circ-SEC31A in NSCLC was analyzed by in situ hybridization on a NSCLC tissue chip (90 cases). (E) Prognostic significance of circ-SEC31A expression for NSCLC patients was performed with FISH values by using the median value as the cut-off; the observation time was 60 months. (F) The genomic loci of the SEC31A gene and circ-SEC31A. N, non-tumor tissues; T, tumor tissues.