Figure 1. The localization of CHK2 during early embryonic development in mice. Embryos at first cleavage were immunolabeled with anti-α-tubulin (green) and anti-CHK2 (red) antibodies, and Hoechst 33342 was used to label DNA (blue). CHK2 was localized near chromosomes after NEBD, and CHK2 accumulated at the spindle area at metaphase, while CHK2 was localized at the spindle poles at anaphase and telophase. Bar = 5 μm.