Figure 4. PVT1 affects the expression and localization of RAB7 in HS766T cells. (A) The mRNA expression of Rab GTPases genes in PVT1-overexpressing HS766T cells. (B) The protein expression of RAB7 in PVT1-overexpressing HS766T cells. (C) Analysis of RAB7 (green) and CD63 (red) in PVT1-overexpressing HS766T cells, as determined by confocal microscope. (D) The interaction between PVT1 and RAB7, as determined by RIP assay and qRT-PCR. (E) The correlation between PVT1 and RAB7, as determined by pull-down assay. (F) The knockdown efficiency of si-RAB7 in HS766T cells. (G) The concentration of exosome derived from HS766T cells with overexpression of PVT1 and knockdown of RAB7. *P < 0.05, data are expressed as the mean ± SD.