Research Paper Volume 12, Issue 11 pp 10427—10440

LncRNA PVT1 promotes exosome secretion through YKT6, RAB7, and VAMP3 in pancreatic cancer

Figure 4. PVT1 affects the expression and localization of RAB7 in HS766T cells. (A) The mRNA expression of Rab GTPases genes in PVT1-overexpressing HS766T cells. (B) The protein expression of RAB7 in PVT1-overexpressing HS766T cells. (C) Analysis of RAB7 (green) and CD63 (red) in PVT1-overexpressing HS766T cells, as determined by confocal microscope. (D) The interaction between PVT1 and RAB7, as determined by RIP assay and qRT-PCR. (E) The correlation between PVT1 and RAB7, as determined by pull-down assay. (F) The knockdown efficiency of si-RAB7 in HS766T cells. (G) The concentration of exosome derived from HS766T cells with overexpression of PVT1 and knockdown of RAB7. *P < 0.05, data are expressed as the mean ± SD.