Research Paper Volume 12, Issue 11 pp 10457—10472

Ellagic acid protects mice against sleep deprivation-induced memory impairment and anxiety by inhibiting TLR4 and activating Nrf2

Figure 9. Experimental design procedure. Mice were randomly divided into four groups after habituation for 7 days. Then, mice were administered EA daily intraperitoneally EA for 21 days. After 3 days of SD habituation (from 8 a.m. to 11 a.m., 3 hours per day), all groups except the control group were subjected to SD for 72 hours (from 8 a.m. on day 18 to 8 a.m. on day 21). Behavioral tests were carried out after 24 hours of SD (Morris water maze training began on day 18).