Research Paper Volume 12, Issue 11 pp 10488—10496

Overexpression of hsa_circ_0136666 predicts poor prognosis and initiates osteosarcoma tumorigenesis through miR-593-3p/ZEB2 pathway

Figure 1. hsa_circ_0136666 overexpression was associated with poor prognosis in OS. (A) hsa_circ_0136666 was upregulated in OS tissues (n=47) compared to normal controls (n=47). (B) hsa_circ_0136666 expression was positively correlated with tumor size. (C) hsa_circ_0136666 expression was higher in advanced stages of OS tissues. (D) hsa_circ_0136666 expression was elevated in OS cell lines. (E) hsa_circ_0136666 overexpression predicted poor prognosis by Kaplan-Meier analysis. (F) Subcellular distribution analysis of hsa_circ_0136666 by qRT-PCR in U2OS and Saos2 cells. *P<0.05 by Student’s t-test or One-way ANOVA. Results from at least three independent experiments were presented as mean ± standard deviation.