Research Paper Volume 12, Issue 11 pp 10488—10496

Overexpression of hsa_circ_0136666 predicts poor prognosis and initiates osteosarcoma tumorigenesis through miR-593-3p/ZEB2 pathway

Figure 3. hsa_circ_0136666 sponged miR-593-3p to upregulate ZEB2. (A) Predicted binding sites between hsa_circ_0136666 and miR-593-3p or between miR-593-3p and ZEB2. (B, C) Luciferase reporter assay using hsa_circ_0136666 or ZEB2 reporter vector in U2OS cells. (D) Expression detection of miR-593-3p after hsa_circ_0136666 knockdown. (E) miR-593-3p mimics inhibited ZEB2 expression. (F) ZEB2 expression was inhibited after hsa_circ_0136666 knockdown. miR-inhibitor: miR-593-3p inhibitor. *P<0.05 by Student’s t-test. Results from at least three independent experiments were presented as mean ± standard deviation.