Research Paper Volume 12, Issue 11 pp 10488—10496

Overexpression of hsa_circ_0136666 predicts poor prognosis and initiates osteosarcoma tumorigenesis through miR-593-3p/ZEB2 pathway


Figure 4. Effects of hsa_circ_0136666/miR-593-3p/ZEB2 axis on OS progression. (A, B) Relative expression of miR-593-3p and ZEB2 in OS tissues. (C) Relative proliferation was measured by CCK8 assay. miR-inhibitor: miR-593-3p inhibitor. (D, E) Migration and invasion were evaluated by Transwell assay. miR-inhibitor: miR-593-3p inhibitor. (F) Apoptosis detection via analysis of Caspase-3/7 activity. miR-inhibitor: miR-593-3p inhibitor. *P<0.05 by Student’s t-test. Results from at least three independent experiments were presented as mean ± standard deviation.