Figure 5. hsa_circ_0136666/miR-593-3p/ZEB2 axis contributes to OS growth in vivo. (A) Tumor size was measured every one week. N=5 for each group. (B) Tumor weight was analyzed after 5 weeks post injection. N=5 for each group. (C–E) Relative expressions of hsa_circ_0136666, miR-593-3p and ZEB2 in tumor tissues were measured by qRT-PCR. (F) Protein levels of Ki67, Caspase3 and N-cadherin were detected by Western blotting. *P<0.05 by Student’s t-test. Results from at least three independent experiments were presented as mean ± standard deviation.