Figure 2. SOCS2-AS1 overexpression inhibited CRC proliferation, migration and invasion. (A) SOCS2-AS1 expression was upregulated in HCT116 and SW480 cells after transfection with SOCS2-AS1 overexpressing vector. (B, C) CCK8 and colony formation assays were performed to determine CRC cell proliferation. (D) Cell proliferation was evaluated by immunofluorescence staining with EdU. (E) Cell-cycle status of CRC cells were analyzed by FACS. (F, G) Transwell assay was performed to analyze cell migration and invasion. *P<0.05.