Research Paper Advance Articles

Human amnion-derived mesenchymal stem cells promote osteogenic differentiation of human bone marrow mesenchymal stem cells via H19/miR-675/APC axis

Figure 1. Osteogenic differentiation of HBMSCs cocultured with HAMSCs, lncRNA-H19 expression in HAMSCs and effects of lncRNA-H19 in HAMSCs on the proliferation of HBMSCs. (A) Relative mRNA expressions of ALP, RUNX2 and OCN in HBMSCs cocultured with HAMSCs were measured by RT-PCR analysis. (B) LncRNA-H19 expression in HAMSCs during coculturing was measured by RT-PCR analysis. (C) HBMSCs proliferation was demonstrated by flow cytometry. Data are shown as mean ± SD. *P < 0.05 and **P < 0.01.