Research Paper Volume 12, Issue 11 pp 10527—10543

Human amnion-derived mesenchymal stem cells promote osteogenic differentiation of human bone marrow mesenchymal stem cells via H19/miR-675/APC axis

Figure 5. MiR-675 downregulates APC expression in HAMSCs. (A) The potential binding sites between APC and miR-675 predicted by biological software. (B) Protein level of APC was assessed by western blot assay in NC, mimics, iNC and inhibitor groups. (C) Relative mRNA expression of APC was measured by RT-PCR analysis in NC, mimics, iNC and inhibitor groups. (D) Luciferase reporter assay was used to validate the target in 293T cells. Relative Renilla luciferase activity was normalized to that of firefly luciferase. Data are shown as mean ± SD. **P < 0.01.