Figure 7. MiR-675 mimic could rescue the shH19 mediated inhibitory effects. (A) Relative mRNA expression of APC was measured by RT-PCR analysis. (B, C) Protein levels of ALP, RUNX2, OCN, OSX, and APC were assessed by western blot assay. (D) Relative mRNA expressions of ALP, RUNX2, OCN, and OSX were measured by RT-PCR analysis. (E) Alizarin red staining analysis. Scale bar, 1 cm. Data are shown as mean ± SD. *P < 0.05 and **P < 0.01.