Human amnion-derived mesenchymal stem cells promote osteogenic differentiation of human bone marrow mesenchymal stem cells via H19/miR-675/APC axis

Xiaojie Ma; Yifeng Bian; Hua Yuan; Ning Chen; Yongchu Pan; Weina Zhou; Shiyu Gao; Xin Du; Shushu Hao; Zixin Yan; Xuan Li; Keyue Liu; Fan Xu; Yuli Wang; Yifei Du

doi:doi:10.18632/aging.103277

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Research Paper Volume 12, Issue 11 pp 10527—10543

Human amnion-derived mesenchymal stem cells promote osteogenic differentiation of human bone marrow mesenchymal stem cells via H19/miR-675/APC axis

Figure 7. MiR-675 mimic could rescue the shH19 mediated inhibitory effects. (A) Relative mRNA expression of APC was measured by RT-PCR analysis. (B, C) Protein levels of ALP, RUNX2, OCN, OSX, and APC were assessed by western blot assay. (D) Relative mRNA expressions of ALP, RUNX2, OCN, and OSX were measured by RT-PCR analysis. (E) Alizarin red staining analysis. Scale bar, 1 cm. Data are shown as mean ± SD. *P < 0.05 and **P < 0.01.