Figure 2. Effect of A2AR antagonist SCH442416 on cellular senescence in vitro. (A) Effect of SCH442416 on cell viability detected by SRB assay. Effect of SCH442416 on cellular senescence detected by (B) cellular senescence markers, SA-β-gal activity and (C) western blot analysis of SCH442416-induced cellular senescence related molecules. Combination test for (D) SCH442416 and BeSO4 as well as (E) SCH442416 and D-gal detected by cellular senescence markers, SA-β-gal activity. Data are mean±SEM from at least four independent experiments. Significant difference between control and treated cells is indicated by *p < 0.05, **p < 0.01.