Research Paper Volume 12, Issue 11 pp 10556—10577

T1-11, an adenosine derivative, ameliorates aging-related behavioral physiology and senescence markers in aging mice

Figure 6. Effect of T1-11 on neurogenesis markers in hippocampus of D-gal-induced aging mice. Hippocampal neurogenesis was detected by immunohistochemical staining with double cortin (DCX, red) and BrdU antibody (green). The full representative immunostaining images and partial enlargement of the dentate gyrus (DG) area are shown in (A, B). (C) The number/mm SGZ of BrdU positive cells (BrdU+) and DCX positive cells (DCX+) are counted. Western blot analysis of (D) neuron aging related molecules and (E) cellular senescence markers. Data are mean±SEM (n = 6). Significant difference between control and D-gal-induced aging mice is indicated by * p < 0.05, ** p < 0.01 compared with the control group. Significant difference between the mice treated with D-gal alone and the mice treated with D-gal combined with Vit.E or T1-11 is indicated by #p < 0.05, ##p < 0.01 compared with D-gal group. 1: Control; 2: D-gal (200 mg/kg); 3: D-gal (200 mg/kg) + Vit.E (100 mg/kg) (positive control); 4: D-galactose (200 mg/kg) + T1-11 (1 mg/kg); 5: D-galactose (200 mg/kg) + T1-11 (10 mg/kg).