Research Paper Volume 12, Issue 11 pp 10578—10593

CARM1 promotes non-small cell lung cancer progression through upregulating CCNE2 expression

Figure 3. CARM1 promotes NSCLC cell proliferation in vitro. (A) Cell proliferation abilities of CARM1-depleted PC9 and HCC827 cells were assessed by CCK-8 assays. The data were presented as means ± SDs of three independent experiments; **P < 0.01. (B) Colony-formative abilities of CARM1-depleted PC9 and HCC827 cells were determined by colony-formation assays. Right panel, the relative colony-formative abilities (% of NC) were quantified. The data were shown as means ± SDs of three independent experiments; **P < 0.01. (C) Overexpression of CARM1 in PC9 and HCC827 cells was examined by Western blot. GAPDH was used as loading control. (D) Cell proliferative abilities of CARM1-overexpressed PC9 and HCC827 cells were assessed by CCK-8 assays. The data were presented as means ± SDs of three independent experiments; **P < 0.01. (E) Colony-formative abilities of CARM1-overexpressed PC9 and HCC827 cells were determined by colony-formation assays. Right panel, the relative colony-formative abilities (% of NC) were quantified. The data were shown as means ± SDs of three independent experiments; **P < 0.01.