Research Paper Volume 12, Issue 12 pp 11530—11549

Hsa_circ_0085576 promotes clear cell renal cell carcinoma tumorigenesis and metastasis through the miR-498/YAP1 axis

Figure 3. Hsa_circ_0085576 promotes cell proliferation, cell cycle, migration and invasion, and inhibits cell apoptosis, in vitro. (A) RT-qPCR analysis of hsa_circ_0085576 levels in A498 cells transfected with LV-sh-hsa_circ_0085576 or LV-shCtrl and in 786O cells transfected with pLVX-hsa_circ_0085576 or pLVK-Ctrl. (B, C) A498 or 786O cell proliferation after the expression of hsa_circ_0085576 was down-regulated or up-regulated, respectively, as assessed by CCK-8 assay. (D, E) A498 cells transfected with LV-sh-hsa_circ_0085576 or LV-shCtrl and 786O cells transfected with pLVX-hsa_circ_0085576 or pLVK-Ctrl were stained by propidium iodide and analyzed using flow cytometry. (F, G) flow cytometry was used to determine the apoptotic rates of A498/LV-sh-circ0085567 or 786O/pLVX-circ0085567. (H, I) the wound-healing assay showed A498 and 786O cell mobility after the expression of hsa_circ_0085576 was down-regulated or up-regulated, respectively. (J, K) Transwell assay showed A498 and 786O cell migration and invasion after the expression of hsa_circ_0085576 was down-regulated or up-regulated, respectively. * P<0.05 vs. LV-sh-Ctrl; ** P<0.05 vs. pLVX-Ctrl.