Research Paper Volume 12, Issue 12 pp 11530—11549

Hsa_circ_0085576 promotes clear cell renal cell carcinoma tumorigenesis and metastasis through the miR-498/YAP1 axis

Figure 5. Hsa_circ_0085576 promotes ccRCC progression through the YAP1 signaling pathway. (A) Differentially expressed genes between the pLVX-Ctrl and pLVX-hsa_circ_0085576 group were shown in the heatmap. Red indicates upregulated; green indicates downregulated. (B) All enriched pathways in Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis with statistical significance. (C) Gene set enrichment analysis (GSEA) showed hsa_circ_0085576 has a significant correlation with Hippo signaling pathway. (D) The mRNA levels of the related genes in the Hippo signaling pathways in 786O cells treated with pLVX-hsa_circ_0085576. (E) Western blot analysis of Hippo signaling pathway (YAP1, LATS1, LATS2, TEAD2 and TEAD3) in A498/LV-sh-hsa_circ_0085576, and 786O/pLVX-hsa_circ_0085576. (F) The double FISH showed that hsa_circ_0085576 and YAP1 were relatively co-localized in the cytoplasm of A498 cells. # P<0.05 vs. pLVX-Ctrl group.