Figure 6. Hsa_circ_0085576 may function as a sponge for miR-498. (A) Venn diagram showing the mutual putative target genes of hsa_circ_0085576 and YAP1 predicted by StarBase and CircInteractome. (B) Dual-luciferase assays showing the luciferase activity of the pmiR-RB-hsa_circ_0085576 vector in 293T cells co-transfected with indicated miRNA mimics. (C–E) Relative expression of miR-498, miR-515-3p and miR-543 in 786O cell infected with pLVX-Ctrl or pLVX-hsa_circ_0085576. (F) A schematic drawing showing the putative miR-498 binding sites with respect to hsa_circ_0085576. (G) RIP experiments were performed using an anti-AGO2 antibody in 786O cells. (H) Hsa_circ_0085576 was inversely correlated with the expression of miR-498 (r = −0.405, P< 0.001) ccRCC tissues. (I, J) Luciferase activity assays were performed in A498 and 786O cells co-transfected with reporter plasmid (or the corresponding mutant reporter) and the indicated miRNAs. (K) Relative expression of miR-498 in A498 cells infected with LV-shCtrl or LV-sh-hsa_circ_0085576. * P<0.05 vs. miR-NC; ** P<0.05 vs. LV-shCtrl; # P<0.05 vs. pLVX-Ctrl.