Research Paper Volume 12, Issue 12 pp 11530—11549

Hsa_circ_0085576 promotes clear cell renal cell carcinoma tumorigenesis and metastasis through the miR-498/YAP1 axis

Figure 7. MiR-498 suppresses YAP1 expression by directly binding to the YAP1 mRNA 3’UTR. (A) The putative binding sites of miR-498 in YAP1 3’-UTR region were predicted. (B) Relative expression of miR-498 in 76 paired ccRCC tissues. (C) miR-498 was inversely correlated with the expression of miR-498 (r = −0.405, P< 0.001) ccRCC tissues. (D, E) Relative expression of YAP1 mRNA in A498 and 786O cells transfected with miR-NC, miR-498 mimics, miR-Ctrl or miR-498 inibitors. (F, G) A498 and 786O cells were transfected with YAP1 wild type reporter or mutant reporter constructs together with miR-498 mimics or inhibitors, and the luciferase activity was analyzed. (H) Western blot analysis of Hippo signaling pathway (YAP1, LATS1, LATS2, TEAD2 and TEAD3) in A498 and 786O cells transfected with miR-NC, miR-498 mimics, miR-Ctrl or miR-498 inibitors. * P<0.05 vs. miR-NC group; ** P<0.05 vs. miR-Ctrl group.