Research Paper Volume 12, Issue 12 pp 11550—11567

Exosome-transmitted long non-coding RNA SENP3-EIF4A1 suppresses the progression of hepatocellular carcinoma

Figure 1. Expression of plasma exosomal SENP3-EIF4A1 in patients with HCC. Exosomes (exos) are isolated from the plasma of HCC patients and healthy controls. (A) Heatmap illustrating the 21 differentially expressed lncRNAs between HCC patients’ plasma exos and healthy control patients’ plasma exos. (B) Detection of SENP3-EIF4A1 in exos from plasma via qRT-PCR. (C) Receiver Operating Characteristic (ROC) curve for the SENP3-EIF4A1 to distinguish HCC cases from controls (Top). Receiver Operating Characteristic (ROC) curve for the SENP3-EIF4A1 to distinguish TNM I/II stage HCC cases from controls (bottom). (D) Determination of SENP3-EIF4A1 in tumor tissues and adjacent normal tissues via qRT-PCR. (E) SENP3-EIF4A1 is lowly expressed in HCC cells compared with HL-7702 cell line. (F) Micrographs and the size distribution of exos isolated from the plasma of HCC patients (left) and healthy controls (right) were detected using TEM (bars =200 nm) and NTA. (G) Detection of TSG101, CD63 and Grp94 in circulating exos via Western blotting. Results are shown as mean ± SD. *P<0.05. All of the experiments were performed in triplicate.