Figure 2. Effect of SENP3-EIF4A1 on HCC cellular phenotype. HuH7 and Hep3b cells are transfected with SENP3-EIF4A1 plasmids or NC vectors. (A) Detection of the mRNA level of SENP3-EIF4A1 via qRT-PCR. (B) Examination of cell viability via CCK8 assays. (C) Determination of cell colony formation ability via a colony-forming growth assay. The colonies are counted and captured. (D) Representative images of migration assays of HuH7 and Hep3b cells (bars =100 μm). The cells are counted. Results are shown as mean ± SD. *P<0.05. All of the experiments were performed in triplicate.