Research Paper Volume 12, Issue 12 pp 11550—11567

Exosome-transmitted long non-coding RNA SENP3-EIF4A1 suppresses the progression of hepatocellular carcinoma

Figure 7. Effects of SENP3-EIF4A1 overexpression and exosomal SENP3-EIF4A1 on tumor in vivo. HuH7 cells are transfected with SENP3-EIF4A1/NC lentiviral vectors, namely SENP3-EIF4A1 vectors and NC, respectively. Exos are separated from HL-7702 cells transfected with SENP3-EIF4A1/NC lentiviral vectors, namely SENP3-EIF4A1-Exos and NC-Exos, respectively. (A) The xenografts from nude mice inoculated in NC, SENP3-EIF4A1-Exos, SENP3-EIF4A1 vectors and NC-Exos (n=5, each group). (B) The tumor volumes are measured every other day after injection. (C) Determination of the tumor weights in nude mice after 15 days. (D) Detection of SENP3-EIF4A1 expression in tumor tissues of nude mice treated with NC, SENP3-EIF4A1 vectors, SENP3-EIF4A1-Exos and NC-Exos by qRT-PCR. (E) Detection of ZFP36 in tumor tissues via Western blotting. (F) Analysis of ZFP36 expression in tumor tissues by IHC. Results are shown as mean ± SD. *P<0.05.