Research Paper Volume 12, Issue 11 pp 10896—10911

SPTBN1 suppresses the progression of epithelial ovarian cancer via SOCS3-mediated blockade of the JAK/STAT3 signaling pathway

Figure 4. Positive correlation between the expression of SPTBN1 and SOCS3 in epithelial ovarian cancer patients and cells. (A, B) Positive correlation between SPTBN1 and SOCS3 expression was investigated by GEPIA (gene expression profiling interactive analysis, http://gepia.cancer-pku.cn) based on the TCGA dataset (A) and observed by IHC staining of ovarian cancer patient samples (n=11) with 40× magnification (B). (C) The protein level of SOCS3 was decreased, and the phosphorylated STAT3 level was increased when SPTBN1 was downregulated. (D) SPTBN1 regulated SOCS3 expression at the mRNA level. * P < 0.05, ** P < 0.01 vs LV-RFP. (E) ChIP-qPCR was performed in HO8910 cells with anti-SPTBN1 and anti-Smads2/3 antibodies to determine the enrichment of SOCS3 promoter region sequences in the obtained ChIP DNA. *P < 0.05 vs Con-si.