Figure 4. The co-existence of APs, ALs and SPs in the brains of APP/PS1/LC3 autophagic flux AD model mice. (A) In the brains of 3-month-old 3×Tg mice, the AP and AL proportion increased, but no SPs were found. In the brains of 6- and 10-month-old 3×Tg mice, SPs coexisted with APs and ALs. Scale bar = 20 μm. (B and C) The quantification of SPs in 3×Tg mice and littoral LC3 mice. (*P < 0.05, **P < 0.01, ***P < 0.001, n=3). (D) Autophagic flux in the different brain regions of 10-month-old 3×Tg mice. There were more APs but less ALs in the cortex and hippocampus, while there were less APs but more ALs in the white matter. Scale bar: a, 500 μm, b-e, 10 μm.