Research Paper Volume 12, Issue 11 pp 10912—10930

Dynamic changes of autophagic flux induced by Abeta in the brain of postmortem Alzheimer’s disease patients, animal models and cell models

Figure 5. The expression of AP and AL in the brains of APP/PS1/LC3 autophagic flux AD model mice. (AC) The APs and ALs in the hippocampus and cortex of 3, 6, 10 -month-old LC3 and 3×Tg mice. Scale bar: a-d, 50 μm, a1-d1, 10 μm, a and c: hippocampus, b and d: cortex, a1-d1 are partial magnifications of a-d. (DK) The number of mRFP and GFP spots in 30 cells from 5 high-power fields was counted. The yellow dots of mRFP and GFP colocalization represent APs, and the free red dots represent ALs. (Compared with LC3 mice, *P<0.05, **P<0.01, ***P<0.001; “ns” denotes that there was no significant difference, n = 3).