Research Paper Volume 12, Issue 11 pp 10931—10950

Hypoxia-preconditioned olfactory mucosa mesenchymal stem cells abolish cerebral ischemia/reperfusion-induced pyroptosis and apoptotic death of microglial cells by activating HIF-1α

Figure 2. OGD/R induced NLRP3 inflammasome activation and pyroptosis in BV2 microglial cells. (A, B) Expression of NLRP3, ASC, pro-caspase1, cleaved-caspase1, pro-caspase8, cleaved-caspase8 and GSDMD in BV2 microglial cells as measured by western-blot analysis. (C, D) Level of IL-1β and IL-18 in BV2 microglial cells as determined by ELISA. (E) The level of LDH activity in BV2 microglial cells as measured by ELISA. All data are presented as the mean value ±SD. *p<0.05; **p<0.01, ***p<0.001, compared with control.