Research Paper Volume 12, Issue 11 pp 10931—10950

Hypoxia-preconditioned olfactory mucosa mesenchymal stem cells abolish cerebral ischemia/reperfusion-induced pyroptosis and apoptotic death of microglial cells by activating HIF-1α

Figure 4. Hypoxia-preconditioned OM-MSCs attenuated cerebral OGD/R-induced pyroptosis in BV2 microglial cells. (A, B) Expression of NLRP3, ASC, pro-caspase1, cleaved-caspase1, pro-caspase8, cleaved-caspase8 and GSDMD in BV2 microglial cells as quantified by Western blotting analysis. (C, D) Level of IL-1β and IL-18 in BV2 microglial cells as measured using ELISA. (E) The level of LDH activity in BV2 microglial cells as measured by ELISA. All data are presented as the mean value ±SD. *p<0.05; **p<0.01, ***p<0.001, compared with normoxia group.