Research Paper Volume 12, Issue 11 pp 10931—10950

Hypoxia-preconditioned olfactory mucosa mesenchymal stem cells abolish cerebral ischemia/reperfusion-induced pyroptosis and apoptotic death of microglial cells by activating HIF-1α

Figure 6. Induction of HIF-1α in OM-MSCs by FG-4592 inhibited cerebral OGD/R-induced apoptosis in BV2 microglial cells. (A, B) The successful overexpression of HIF-1α in OM-MSCs was confirmed by Western blotting. (C, E) The apoptosis rate of BV2 microglial cells was determined by flow cytometry with Annexin V/PI staining in each group. (D, F) Production of ROS levels in BV2 microglial cells was measured by flow cytometry. (G, H) The protein expression of caspase3 in BV2 microglial cells was quantified by Western blotting analysis. (I) The viability of BV2 microglial cells was evaluated with MTT assay. All data are presented as the mean value ±SD. *p<0.05; **p<0.01, ***p<0.001, compared with normoxia group or hypoxia group.