Figure 10. Sal promotes increases in dendritic spine density and synaptic-associated protein expression via upregulating the FGF2-mediated cAMP/PKA/CREB signaling pathway. (A) Examples of dendritic spines (the scale bar is 10 μm). (B, C) Density of dendritic spines. (D) Double immunofluorescence staining of PSD95-positive (green) and NeuN-positive (red) neurons from sections in each group on day 7 after MCAO/R (the scale bars are 20 and 10 μm). (E–H) Representative western blot bands of PSD95, synapsin I and synaptotagmin in each group. (I) QPCR data for PSD95. (J) Transmission electron microscopy showed the synaptic structures (the scale bar is 1 μm). Values are expressed as the mean ± SD. #p < 0.05, ##p < 0.01 vs. sham; *p < 0.05, **p < 0.01 vs. MCAO/R.