Research Paper Volume 12, Issue 11 pp 10983—11003

The circRNA circIFI30 promotes progression of triple-negative breast cancer and correlates with prognosis

Figure 7. circIFI30 promotes cell proliferation, migration and invasion through circIFI30/miR-520b-3p/CD44 axis. (A) The cell viability was determined after transfection with indicated vectors, miR-520b-3p mimics or inhibitors by CCK8 assay. (B, C) The cell proliferation was detected after transfection with indicated vectors, miR-520b-3p mimics or inhibitors by EdU assay (magnification, × 100, scale bar, 100 μm). (D, E) The cell survival was measured after transfection with indicated vectors, miR-520b-3p mimics or inhibitors by colony formation assay. (F, G) The cell migration capacity was detected after transfection with indicated vectors, miR-520b-3p mimics or inhibitors by wound healing assays (magnification, × 50). Scale bar, 200 μm. (H, I) The cell invasion ability was determined after transfection with indicated vectors, miR-520b-3p mimics or inhibitors by transwell assays (magnification, × 100, scale bar, 100 μm). (J, K) Relative expressions of CD44 and EMT-related molecules at protein level in cells transfected with indicated vectors, miR-520b-3p mimics or inhibitors were determined by western blot. Data were indicated as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001.