Research Paper Volume 12, Issue 11 pp 11004—11024

Upregulation of Mlxipl induced by cJun in the spinal dorsal horn after peripheral nerve injury counteracts mechanical allodynia by inhibiting neuroinflammation

Figure 6. Mlxipl inhibited the cJun-mediated inflammatory response in microglia. (AF) The Mlxipl expression was detected by QPCR (AB), western blot (E) and immunofluorescence (E). The primary microglia were co-transfected with siMlxipl or LvMlxipl for 48 hr, and then treated with LPS (1 μg/ml) for 24 hr. Quantification of the western blot (D) and immunofluorescence (F). Data relative to vehicle. N = 3. $$$P < 0.001, $$P < 0.01, $P < 0.05 vs. LPS; ###P < 0.001, ##P < 0.01 vs. LPS-siNC; ***P < 0.001, **P < 0.01, *P < 0.05 vs. LPS-LvNC. (GI) ELISA were performed to detect the expression of proinflammation cytokines. Mlxipl inhibited inflammation response in LPS-induced microglia. N = 3. $$$P < 0.001, $$P < 0.01, $P < 0.05 vs. LPS; ###P < 0.001, ##P < 0.01 vs. LPS-siNC; ***P < 0.001, **P < 0.01, *P < 0.05 vs. LPS-LvNC. (JO) The cJun expression was detected by QPCR (J–K), western blot (L) and immunofluorescence (N). The primary microglia were co-transfected with sicJun or LvcJun for 48 hr, and then treated with LPS (1 μg/ml) for 24 hr. Knockdown of cJun inhibited the expression of Mlxipl and p-cJun. Overexpression of cJun promoted the expression of Mlxipl and p-cJun. Quantification of the western blot (M) and immunofluorescence (O). Data relative to vehicle. N = 3. $$$P < 0.001, $$P < 0.01, $P < 0.05 vs. LPS; ###P < 0.001, ##P < 0.01 vs. LPS-siNC; ***P < 0.001, **P < 0.01, *P < 0.05 vs. LPS-LvNC. (PR) ELISA were performed to detect the expression of proinflammation cytokines. CJun promoted inflammation response in LPS-induced microglia. N = 3. $$$P < 0.001, $$P < 0.01, $P < 0.05 vs. LPS; ###P < 0.001, ##P < 0.01 vs. LPS-siNC; ***P < 0.001, **P < 0.01, *P < 0.05 vs. LPS-LvNC.