Figure 7. The role of LncRNA ADAMTS9/miR-223-3p axis in the regulation of pyroptotic cell death in cisplatin treated CR-GC cells in vitro and in vivo. Western Blot was used to detect the expression levels of NLRP3 and ASC in (A, B) CR-SGC7901 cells and (C, D) CR-BGC-823 cells. (E) The activity of Caspase-1 was determined in CR-GC cells. ELISA was employed to examine (F) IL-1β and (G) IL-18 expressions in the supernatants. (H) LDH release was measured to evaluate the formation of pores in the cell membrane. (I) LDH release was measured in mice cancer tissues. (J, K) The expressions of NLRP3 and ASC were determined by Western Blot in mice cancer tissues. (L) ELISA was used to measure IL-1β and IL-18 expressions in mice serum. (M) Caspase-1 activity was measured in mice cancer tissues. Each experiment repeated at least 3 times. “NS” represented “no statistical significance”, *P < 0.05, **P < 0.01.