Figure 3. Confirmation of monoclonal PTX-resistant cells. (A) Cell viability curve. The viability of OVCAR-3 and OV3R-PTX-B4 cells that resisted to PTX were evaluated by the CCK-8 assay. OVCAR-3 and OV3R-PTX-B4 cells (4000 cells/well) were treated with PTX in a dose-dependent study (0.001 0.01, 0.1, 1, 2, 5, 10, and 25 μM/ml) for 48 h. (B) Capacity of spheroid formation. OVCAR-3 and OV3R-PTX-B4 cells were cultured in serum-free DMEM/F12 medium with EGF, bFGF, heparin, and B27 supplements under a low-adhesive condition for 11 days. The pictures were taken by phase-contrast microscopy every 2 days. Representative images are shown. (C) Quantitative analysis of spheroid diameter from B. n = 3 independent experiments; *, P < 0.05; **, P < 0.01.