Figure 5. Expression of stemness markers in OV3R-PTX-B4. Cells were cultured under a monolayer culture system (Mono) or a spheroid culture system (Sph). The expression of CD44, CD133, NANOG, and OCT4 proteins were detected by Western blot. (A) CD44 expression. (B) CD133 expression. (C) NANOG expression. (D) OCT4 expression. Upper panel, representative images of blotting; low panel, semi-quantitative analysis of the relative optical density of protein bands in the upper panel. β-tubulin and GAPDH were used as loading controls. n = 3; **, P < 0.01.