Figure 7. Effect of STAT1-siRNA on stemness markers in OV3R-PTX-B4 cells. Cells were transfected with STAT1-siRNA (STAT1-siR) or negative control (NC) scramble siRNA. The expression of STAT1, CD44, CD133, NANOG, and OCT4 proteins were detected by Western blot. (A) STAT1 expression. (B) CD44 expression. (C) CD133 expression. (D) NANOG expression. (E) OCT4 expression. Upper panel, representative images of blotting; low panel, semi-quantitative analysis of the relative optical density of protein bands in the upper panel. GAPDH was used as a loading control. n = 3; *, P < 0.05; **, P < 0.01.