Research Paper Volume 12, Issue 11 pp 11061—11070

T cell stimulation and expansion by SunTag-based clustering of anti-CD3/CD28 scFv

Figure 3. Expansion of T cells from aged cancer patients. (A) Representative images of T cells from aged cancer patients cultured with CD3/CD28 antibody or SBCS (30 nM 13×αCD3scFv/10 nM 13×αCD28scFv). (B) Fold expansion of T cells from aged cancer patients treated with immobilized CD3/CD28 antibodies or SBCS for 14 days. (C, D) CD4-to-CD8 ratio of CD4+ and CD8+ single-positive cells among live cells after treatment with CD3/CD28 immobilized antibodies or SBCS for 14 days. ‘d0’ represents peripheral blood mononuclear cells before cell expansion. Data in B–D represent mean ± s.d. of n = 3 aged cancer patients and are representative of at three independent experiments. *** P < 0.001. ns, not significant. Scale bars = 100 μm.