Research Paper Volume 12, Issue 12 pp 11579—11602

Long-term PM2.5 exposure increases the risk of non-small cell lung cancer (NSCLC) progression by enhancing interleukin-17a (IL-17a)-regulated proliferation and metastasis

Figure 2. PM2.5 treatments lead to cancer stem cell properties in mice. (A) RT-qPCR analysis was used to measure lung cancer-related biomarkers (Kras, c-Myc, ABCG2, OCT4, SOX2, Aldh1a1, p53 and PTEN) in lung tissues of mice following PM2.5 treatment at the indicated time (n = 4). (B) IHC staining was performed to calculate c-Myc, OCT4 and SOX2 in pulmonary sections of PM2.5-challenged mice (n = 6). The quantification of c-Myc, OCT4 and SOX2 relative expression was exhibited. Scale bar, 100 μm. All data are expressed as mean ± SEM. *p<0.05 and **p<0.01 compared to the FA group.