Figure 3. PM2.5 enhances IL-17a expression in mice. (A, B) IL-17a contents in BALF and serum were measured by ELISA, respectively (n = 8). (C) RT-qPCR, (D) western blotting and (E) IHC analysis of IL-17a in lung tissues of mice challenged with PM2.5 for the indicated time (n = 6). Scale bar, 100 μm. (F, G) IL-17a levels in the peripheral blood lymphocytes and in splenic lymphocytes were calculated using ELISA analysis (n = 8). All data are expressed as mean ± SEM. *p<0.05, **p<0.01 and ***p<0.001 compared to the FA group.