Research Paper Volume 12, Issue 12 pp 11603—11622

Over-expression of microRNA-145 drives alterations in β-adrenergic signaling and attenuates cardiac remodeling in heart failure post myocardial infarction


Figure 6. miR-145 increased ICa density and reduced the sensitivity of HF to β-adrenergic stimulation. (A) representative ICa traces recording; (B) ICa-voltage relationship of cardiomyocytes (n≥7); (C) summary of peak ICa (n≥7); (D) the steady-state ICa activation curves (n≥7); (E) the steady-state ICa inactivation curves (n≥7); (F) the recovery curves following inactivation (n≥7); (G) representative western blots of Cav1.2, p-CaMKII and CaMKII; (HJ) quantitative analysis of the immunoreactive band displayed by bar graph (n=3); (K) ICa-voltage relationship in presence of ISO for each group (n≥7); (L) summary of peak ICa in the presence of ISO (n≥7); (M) changes of peak ICa for each group (n≥7); (A) sham group; (B) HF group; (C) AD-EGFP; (D) AD-miR-145 group; (E) M+C group. Data are presented as mean ± SD for C, H, I, J, L and M. While for B, D, E, F and K, data are presented as mean ± SEM.