Research Paper Volume 12, Issue 11 pp 11071—11084

LINC01419 facilitates hepatocellular carcinoma growth and metastasis through targeting EZH2-regulated RECK

Figure 3. LINC01419 silences RECK epigenetically by binding to EZH2. (A) The different gene transcripts expression between si-ctrl cells and si- LINC01419 cells, demonstrated by hierarchical cluster. (B) The promoter regions of RECK showing EZH2 transcriptional sites, as indicated by UCSC. (C) LINC01419 interaction with EZH2, as revealed by the RIP experiments. (D) Desthiobiotinylation-LINC01419 bound EZH2 in HCC cells, as indicated by the pull-down assays.(E)Shows EZH2 down-regulation by si-RNA in HCC cells, and the knockdown efficiency examination using western blot assay. (F) qPCR assay examination of the mRNA expression level of RECK. (G) The western blot analysis of the RECK protein expression level. (H) Showing EZH2 and H3K27me3 enriched in the RECK promoter regions as indicated by CHIP assay. (I) Sowing increased EZH2 and H3K27me3levelsafter LINC01419 overexpression in HCC cell.