Research Paper Volume 12, Issue 11 pp 11071—11084

LINC01419 facilitates hepatocellular carcinoma growth and metastasis through targeting EZH2-regulated RECK

Figure 5. RECK suppresses HCC cell proliferation and metastasis, and counteracts LINC01419 activity. (A) RT-PCR assay to examine the RECK expression level. (B) The mRNA level of RECK as examined by qRT-PCR. (C) The RECK protein level as examined by western blot assay. (DF) Analysis of cells by MTT assays (D), colony formation (E), and transwell assays (F) (G) Western blot assay was performed to examine E-cadherin, N-cadherin and Vimentin expression levels.