Figure 6. The GnRH expression levels in the hypothalamic arcuate nucleus. (A) GnRH positive expression in the brown area. The scale bar = 100 um. (B) The statistics of the GnRH average optical density value of each group. Image-Pro Plus 6.0 software was used to calculate the average optical density of GnRH. vs control group, ▲▲p < 0.01. vs HFD group, * p < 0.05, ** p < 0.01. vs DC group, #p < 0.05, ##p < 0.01.