Research Paper Volume 12, Issue 11 pp 11100—11115

ANXA7 promotes the cell cycle, proliferation and cell adhesion-mediated drug resistance of multiple myeloma cells by up-regulating CDC5L

Figure 10. CDC5L interference inhibits the cell cycle promotion effect of ANXA7. (A) The images of flow cytometry for U266 and RPMI8226 cells after transfection of shRNA-CDC5L. (B) The cell cycle distribution of U266 and RPMI8226 cells after transfection of shRNA-CDC5L was analyzed by flow cytometry analysis. *P<0.05 and ***P<0.001 vs.U266 group. ##P<0.01 and ###P<0.001 vs. U266-OE-ANXA7 group. ΔΔP<0.01 and ΔΔΔP<0.001 vs. RPMI8226 group. $$$P<0.001 vs. RPMI8226-OE-ANXA7 group. (C) The protein expression of CDK1 and cyclinB1 in U266 and RPMI8226 cells after transfection of shRNA-CDC5L was detected by Western blot analysis. **P<0.01 and ***P<0.001 vs.U266 group. ###P<0.001 vs. U266-OE-ANXA7 group. ΔΔΔP<0.001 vs. RPMI8226 group. $P<0.05 and $$P<0.01 vs. RPMI8226-OE-ANXA7 group.