Research Paper Volume 12, Issue 11 pp 11100—11115

ANXA7 promotes the cell cycle, proliferation and cell adhesion-mediated drug resistance of multiple myeloma cells by up-regulating CDC5L

Figure 3. ANXA7 overexpression accelerates the cycle of U266 and RPMI8226 cells. (A) The images of flow cytometry for U266 and RPMI8226 cells after transfection of OE-ANXA7. (B) The cell cycle distribution of U266 and RPMI8226 cells after transfection of OE-ANXA7 was analyzed by flow cytometry analysis. ***P<0.001 vs.U266 group. #P<0.05 and ###P<0.001 vs. U266+OE-NC group. ΔΔΔP<0.001 vs. RPMI8226 group. $$$P<0.001 vs. RPMI8226-NC group. (C) The protein expression of CDK1 and cyclinB1 in U266 and RPMI8226 cells after transfection of OE-ANXA7 was detected by Western blot analysis. **P<0.01 and ***P<0.001 vs.U266 group. ##P<0.01 and ###P<0.001 vs. U266-OE-NC group. ΔΔΔP<0.001 vs. RPMI8226 group. $$$P<0.001 vs. RPMI8226-OE-NC group.