Research Paper Volume 12, Issue 11 pp 11139—11151

Melatonin regulates mitochondrial dynamics and alleviates neuron damage in prion diseases

Figure 1. Melatonin attenuated PrP106-126-induced N2a cell apoptosis. (A) N2a cell viability was assayed using the CCK8 kit after treatment with melatonin and PrP106-126. (B, C) N2a cell apoptosis was assayed by TUNEL staining. (DG) Protein expression of cleaved caspase-9, cleaved caspase-3 and Bcl2 in N2a cells, and protein expression of cytochrome c and Bax in cytosolic and mitochondrial extracts of N2a cells by western blotting. *P < 0.05, **P < 0.01, ***P < 0.001. All experiments were repeated at least three times.